Human G6B HEK293 Overexpression Lysate: Product Information
This Human G6B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of G6B protein (Cat: 11273-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human G6B (NP_612116.1) (Met1-Gln146) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human G6B consists 363 amino acids and predicts a molecular mass of 40.2 kDa.
Human G6B HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Successfully added to cart Please enter catalog numberSubmitted successfullyNetwork ErrorPlease enter your company namePlease enter your namePlease enter your emailPlease enter a valid email addressPlease enter some messageNot found.