Human DcR1/TRAILR3 HEK293 Overexpression Lysate: Product Information
This Human DcR1/TRAILR3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DcR1/TRAILR3 protein (Cat: 10415-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human TNFRSF10C (NP_003832.2) (Met1-Pro235) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human TNFRSF10C consists of 221 amino acids and predicts a molecular mass of 23.6 kDa.
Human DcR1/TRAILR3 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human DcR1/TRAILR3 HEK293 Overexpression Lysate: Alternative Names
Human CD263 Overexpression Lysate; Human DCR1 Overexpression Lysate; Human DCR1-TNFR Overexpression Lysate; Human LIT Overexpression Lysate; Human TRAIL-R3 Overexpression Lysate; Human TRAILR3 Overexpression Lysate; Human TRID Overexpression Lysate
DcR1/TRAILR3 Background Information
TNFRSF1C CNV in patients with CRC is associated with distant metastatic disease. A high frequency of CGI methylation in the TNFRSF1C promoter results in inactivation of the gene and enhancement of tumor growth in most PC cell lines (except CFPAC-1). Inactivation of TNFRSF1C by CpG island (CGI) hypermethylation can play an important role in PC progression and be potentially useful as a diagnostic marker and a new therapeutic approach for PC.
tumor necrosis factor receptor superfamily member 10c
Successfully added to cart Please enter catalog numberSubmitted successfullyNetwork ErrorPlease enter your company namePlease enter your namePlease enter your emailPlease enter a valid email addressPlease enter some messageNot found.