Human Placental Lactogen/CSH1 HEK293 Overexpression Lysate: Product Information
This Human Placental Lactogen/CSH1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Placental Lactogen/CSH1 protein (Cat: 11596-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human CSH1 (NP_001308.1) (Met 1-Phe 217) was expressed, fused with a polyhistidine tag at the C-terminus.
The secreted recombinant human CSH1 consists of 202 amino acids and has a calculated molecular mass of 23.7 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
Human Placental Lactogen/CSH1 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Placental Lactogen/CSH1 HEK293 Overexpression Lysate: Alternative Names
Human CS-1 Overexpression Lysate; Human CSA Overexpression Lysate; Human CSMT Overexpression Lysate; Human hCS-1 Overexpression Lysate; Human hCS-A Overexpression Lysate; Human PL Overexpression Lysate
Placental Lactogen/CSH1 Background Information
Chorionic somatomammotropin hormone, also known as Choriomammotropin, Lactogen, Placental lactogen and CSH1, is a secreted protein which belongs to thesomatotropin / prolactin family. CSH1 is produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization. The CSH1 gene is member of the GH gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. Genomic alterations in the GH cluster are well known, causing different phenotypes depending on the size of the deletion and the genes involved. The increased prevalence of hemizygosity of CSH1 in population in comparison to controls indicates a role for CSH1 haploinsufficiency in the etiology of growth retardation. Investigation of CSH1 deletions in further SRS and growth retarded patients will enable us to establish under which circumstances haploinsufficiency of CSH1 is likely to result in clinical changes.
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