Human CD300f/CD300LF HEK293 Overexpression Lysate

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Human CD300f/CD300LF HEK293 Overexpression Lysate: Product Information

Product Description
This Human CD300f/CD300LF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD300f/CD300LF protein (Cat: 16034-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human CD300LF (NP_620587.2) (Met1-Leu155) was expressed with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human CD300LF consists of 147 amino acids and predicts a molecular mass of 16.9 kDa.

Human CD300f/CD300LF HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human CD300f/CD300LF HEK293 Overexpression Lysate: Alternative Names

Human CD300f Overexpression Lysate; Human CLM-1 Overexpression Lysate; Human CLM1 Overexpression Lysate; Human IgSF13 Overexpression Lysate; Human IREM-1 Overexpression Lysate; Human IREM1 Overexpression Lysate; Human LMIR3 Overexpression Lysate; Human NKIR Overexpression Lysate

CD300f/CD300LF Background Information

Full Name
CD300 molecule-like family member f
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