Human CD204/MSR1 HEK293 Overexpression Lysate: Product Information
This Human CD204/MSR1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD204/MSR1 protein (Cat: 10427-H07H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain (Lys 77-Leu 451) of human scavenger receptor 1 isoform type 1 (NP_619729.1) was fused with the polyhistidine tag at the N-terminus.
The recombinant human MSR1 consisting of 391 amino acids predicts a molecular mass of 43 kDa. Due to glycosylation, apparent molecular mass of rh MSR1 is approximately 55-65 kDa in SDS-PAGE under reducing conditions.
Human CD204/MSR1 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CD204/MSR1 HEK293 Overexpression Lysate: Alternative Names
Human CD204 Overexpression Lysate; Human phSR1 Overexpression Lysate; Human phSR2 Overexpression Lysate; Human SCARA1 Overexpression Lysate; Human SR-A Overexpression Lysate; Human SRA Overexpression Lysate
CD204/MSR1 Background Information
Macrophage scavenger receptor types I and II, also known as Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1, CD24, MSR1 and SCARA1, is a single-pass type II membrane protein which contains onecollagen-like domain and oneSRCR domain. Macrophages are distributed in all peripheral tissues and play a critical role in the first line of the innate immune defenses against bacterial infection by phagocytosis of bacterial pathogens through the macrophage scavenger receptor 1 (MSR1). MSR1 / SCARA1 is one of the membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). MSR1 / SCARA1 is also involved in chronic inflammation which is a risk factor for prostate cancer. MSR1 1 gene was identified as a candidate susceptibility gene for hereditary prostate cancer and as a risk factor for sporadic prostate cancer.
macrophage scavenger receptor 1
Wang L. et al., 2003, Nat Genet. 35 (2): 128-9.
Chen Y.C. et al., 2008, Cancer Epidemiol Biomarkers Prev. 17 (4): 1001-3.
Shirato K. et al., 2009, Pflugers Arch. 459 (1): 93-103.
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