Human CD122/IL2RB HEK293 Overexpression Lysate: Product Information
This Human CD122/IL2RB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD122/IL2RB protein (Cat: 10696-HCCH) from the overexpression lysate was verified.
The mature form of human IL2 Rβ (NP_000869.1) extracellular domain (Met 1-Asp 239) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
The mature recombinant human IL2 Rβ consists of 219 amino acids and has a predicted molecular mass of 25.2 kDa. As a result of glycosylation, the rh IL2 Rβ migrates as an approximately 40 kDa protein in SDS-PAGE under reducing conditions.
Human CD122/IL2RB HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human CD122/IL2RB HEK293 Overexpression Lysate: Alternative Names
Human CD122 Overexpression Lysate; Human IL-15RB Overexpression Lysate; Human IL15RB Overexpression Lysate; Human P70-75 Overexpression Lysate
CD122/IL2RB Background Information
Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B22+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.
interleukin 2 receptor, beta
Foss F. (2006) Clinical experience with denileukin diftitox (ONTAK). Semin Oncol. 33(1 Suppl 3): 11-6.
Sprent J, et al. (2001) T cell death and memory. Science. 293(5528): 245-8.
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