Human Casein Kinase 1 alpha Baculovirus-Insect cells Overexpression Lysate: Product Information
This Human Casein Kinase 1 alpha overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Casein Kinase 1 alpha protein (Cat: 10668-H09B) from the overexpression lysate was verified.
A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.
The recombinant human CSNK1A1/GST chimera consists of 561 amino acids and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Casein Kinase 1 alpha Baculovirus-Insect cells Overexpression Lysate: Alternative Names
Human CK1 Overexpression Lysate; Human CK1a Overexpression Lysate; Human CKIa Overexpression Lysate; Human HEL-S-77p Overexpression Lysate; Human HLCDGP1 Overexpression Lysate; Human PRO2975 Overexpression Lysate
Casein Kinase 1 alpha Background Information
Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.
casein kinase 1, alpha 1
Dubois, et al.,2002, FEBS Lett. (Netherlands) 517 (1-3): 167-71.
Dubois, T et al.,2001, J. Biol. Chem.(United States) 276 (22): 18757-64.
Zhang, Yi et al., 2002, J. Biol. Chem. 277(20): 17706-12.
Wang,Z. et al., 2010, Med Sci Monit.16 (8):CR357-64.
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