Anti-Leukotriene A4 Hydrolase Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Leukotriene A4 Hydrolase Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Leukotriene A4 Hydrolase Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Leukotriene A4 Hydrolase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Leukotriene A4 Hydrolase Magnetic Beads-IP Kit Product Description

The Anti-Leukotriene A4 Hydrolase magnetic Beads, conjugated with Anti-Leukotriene A4 Hydrolase antibody, are used for immuneprecipitation (IP) of Leukotriene A4 Hydrolase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Leukotriene A4 Hydrolase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Leukotriene A4 Hydrolase proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Leukotriene A4 Hydrolase Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Leukotriene A4 Hydrolase Antibody(50268-RP02)
Immunogen
Recombinant Mouse LTA4H protein (Catalog#50268-M08B)
Species Reactivity
Mouse LTA4H / LTA4 Hydrolase
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse LTA4H / LTA4 Hydrolase (rM LTA4H; Catalog#50268-M08B; NP_032543.2; Met 1-Asp 611). LTA4H specific IgG was purified by mouse LTA4H affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Leukotriene A4 Hydrolase Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-Lta4hALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Leukotriene A4 Hydrolase Background Information

Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to thepeptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
Full Name
leukotriene A4 hydrolase
References
  • Mancini, JA. et al.,1995, Eur. J. Biochem. 231: 65-71.
  • Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73.
  • Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82.
  • Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
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