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Anti-LRPAP1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-LRPAP1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-LRPAP1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-LRPAP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-LRPAP1 Magnetic Beads-IP Kit Product Description

The Anti-LRPAP1 magnetic Beads, conjugated with Anti-LRPAP1 antibody, are used for immuneprecipitation (IP) of LRPAP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing LRPAP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound LRPAP1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-LRPAP1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-LRPAP1 Antibody(11100-M105)
Immunogen
Recombinant Human LRPAP1 protein (Catalog#11100-H08H)
Species Reactivity
Human LRPAP1
Source
Monoclonal Human Mouse IgG1
Preparation
This antibody was obtained from a mouse immunized with purified, recombinant Human LRPAP1 (rh LRPAP1; Catalog#11100-H08H; NP_002328.1; Tyr35-Leu357).
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-LRPAP1 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-A2MRAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-A2RAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-alpha-2-MRAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HBP44ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MRAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MYP23ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

LRPAP1 Background Information

Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 33), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiosis. The genetic variations at the LRPAP1 locus may modulate Alzheimer disease (AD) phenotype beyond risk for disease. In addition, the variation at the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).
Full Name
low density lipoprotein receptor-related protein associated protein 1
References
  • Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.
  • Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19?
  • Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.
  • Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.
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