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Anti-IL11RA Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-IL11RA Magnetic Beads-IP Kit Product Components

Components Storage
Anti-IL11RA Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-IL11RA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IL11RA Magnetic Beads-IP Kit Product Description

The Anti-IL11RA magnetic Beads, conjugated with Anti-IL11RA antibody, are used for immuneprecipitation (IP) of IL11RA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL11RA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL11RA proteins are dissociated from the magnetic beads using an elution buffer.

Anti-IL11RA Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-IL11RA Antibody(10252-T52)
Immunogen
Recombinant Human IL11RA / IL-11RA Protein (Catalog#10252-H08H)
Species Reactivity
Human IL11RA / IL-11RA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IL11RA / IL-11RA (rh IL11RA / IL-11RA; Catalog#10252-H08H; NP_004503.1; Met1-Val363). IL11RA / IL-11RA specific IgG was purified by Human IL11RA / IL-11RA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-IL11RA Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CRSDAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-hCG_2011440ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-IL11RAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MGC2146ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

IL11RA Background Information

Interleukin 11 receptor, alpha subunit (IL11RA/IL-11RA) is a subunit of the interleukin 11 receptor which is a member of the hematopoietic cytokine receptor family. IL11RA/IL-11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Alternative splicing has been observed at this locus, and three variants encoding two different isoforms have been identified. IL11RA/IL-11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. Defects in IL11RA/IL-11RA are a cause of craniosynostosis and dental anomalies (CRSDA). CRSDA is a disorder characterized by craniosynostosis, maxillary hypoplasia, and dental anomalies, including malocclusion, delayed and ectopic tooth eruption, and/or supernumerary teeth. Some patients also display minor digit anomalies, such as syndactyly and/or clinodactyly.
Full Name
interleukin 11 receptor subunit alpha
References
  • Van Leuven F, et al. (1996) Molecular cloning and characterization of the human interleukin-11 receptor alpha-chain gene, IL11RA, located on chromosome 9p13. Genomics. 31 (1): 65-70.
  • Yoshizaki A, et al. (2006) Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells. Int J Oncol. 29 (4): 869-76.
  • Karube K, et al. (2006) Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed-Sternberg (HRS) cells: high expression of interleukin-11 receptor alpha. Ann Oncol. 17 (1): 110-6.
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