Anti-HtrA2/Omi Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-HtrA2/Omi Magnetic Beads-IP Kit Product Components

Components Storage
Anti-HtrA2/Omi Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-HtrA2/Omi magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-HtrA2/Omi Magnetic Beads-IP Kit Product Description

The Anti-HtrA2/Omi magnetic Beads, conjugated with Anti-HtrA2/Omi antibody, are used for immuneprecipitation (IP) of HtrA2/Omi proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HtrA2/Omi proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HtrA2/Omi proteins are dissociated from the magnetic beads using an elution buffer.

Anti-HtrA2/Omi Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-HtrA2/Omi Antibody(10619-T60)
Immunogen
Recombinant Human HtrA2 Protein (Catalog#10619-H08E)
Species Reactivity
Human HtrA2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human HtrA2 (rh HtrA2; Catalog#10619-H08E; O43464-1; Ala134-Glu458). HtrA2 specific IgG was purified by Human HtrA2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-HtrA2/Omi Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-OMIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PARK13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRSS25ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

HtrA2/Omi Background Information

Serine protease HTRA2, also known as high temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein which belongs to thepeptidase S1B family. HTRA2 contains onePDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa.
Full Name
HtrA serine peptidase 2
References
  • Faccio L., et al., 2000, J. Biol. Chem. 275:2581-2588.
  • Gray C.W., et al., 2000, Eur. J. Biochem. 267:5699-5710.
  • Suzuki Y., et al., 2001, Mol. Cell 8:613-621.
  • Strauss K.M., et al., 2005, Hum. Mol. Genet. 14:2099-2111.
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