Anti-Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Ebola virus VP40 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Ebola virus VP40 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Ebola virus VP40 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Ebola virus VP40 Magnetic Beads-IP Kit Product Description

The Anti-Ebola virus VP40 magnetic Beads, conjugated with Anti-Ebola virus VP40 antibody, are used for immuneprecipitation (IP) of Ebola virus VP40 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Ebola virus VP40 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Ebola virus VP40 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Ebola virus VP40 Magnetic Beads-IP Kit Antibody Information

Antibody
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix Antibody, Rabbit PAb, Antigen Affinity Purified(40448-T52)
Immunogen
Recombinant Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix Protein (Catalog#40448-V07E)
Species Reactivity
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix
Source
Polyclonal EBOV Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix ( Catalog#40448-V07E; YP_003815434.1; Ala31-Lys326). Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix specific IgG was purified by Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
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