Anti-DDR1 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-DDR1 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-DDR1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-DDR1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-DDR1 Magnetic Beads-IP Kit Product Description

The Anti-DDR1 magnetic Beads, conjugated with Anti-DDR1 antibody, are used for immuneprecipitation (IP) of DDR1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing DDR1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound DDR1 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-DDR1 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-DDR1 Antibody(10730-R203)
Immunogen
Recombinant Human DDR1 / CD167 protein (Catalog#10730-H08H)
Species Reactivity
Human DDR1 / CD167
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human DDR1 / CD167 (rh DDR1 / CD167; Catalog#10730-H08H; NP_001945.3; Met 1-Thr 416).
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-DDR1 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CAKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CD167ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-DDRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-EDDR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HGK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MCK10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NEPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NTRK4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PTK3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PTK3AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RTK6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TRKEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

DDR1 Background Information

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 1 (MCK1), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK1/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK1/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
Full Name
discoidin domain receptor tyrosine kinase 1
Related Pathways
  • p53 Pathway
    p53 Pathway
References
  • Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
  • Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
  • Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
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