Anti-MERS-CoV (NCoV / Novel coronavirus) Spike Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.
Price:
Size:
Number:

Anti-Coronavirus spike Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Coronavirus spike Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Coronavirus spike magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Coronavirus spike Magnetic Beads-IP Kit Product Description

The Anti-Coronavirus spike magnetic Beads, conjugated with Anti-Coronavirus spike antibody, are used for immuneprecipitation (IP) of Coronavirus spike proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Coronavirus spike proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Coronavirus spike proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Coronavirus spike Magnetic Beads-IP Kit Antibody Information

Antibody
MERS-CoV Spike Antibody, Rabbit PAb, Antigen Affinity Purified(40069-T44)
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (Catalog#40069-V08B)
Species Reactivity
MERS-CoV (NCoV / Novel coronavirus) Spike
Source
Polyclonal MERS-CoV Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Spike (Catalog#40069-V08B; AFS88936.1; Met1-Trp1297). MERS-CoV (NCoV / Novel coronavirus) Spike specific IgG was purified by MERS-CoV (NCoV / Novel coronavirus) Spike affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Citation List

Anti-MERS-CoV (NCoV / Novel coronavirus) Spike Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-coronavirus s1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-coronavirus s2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-coronavirus spikeALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-cov spikeALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ncov RBDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ncov s1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ncov s2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ncov spikeALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RBDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-s1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Spike RBDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Coronavirus spike Background Information

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
References
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.