Anti-CHEK2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CHEK2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CHEK2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CHEK2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CHEK2 Magnetic Beads-IP Kit Product Description

The Anti-CHEK2 magnetic Beads, conjugated with Anti-CHEK2 antibody, are used for immuneprecipitation (IP) of CHEK2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CHEK2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CHEK2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CHEK2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CHEK2 Antibody(50432-RP02)
Immunogen
Recombinant Mouse CHK2 / CHEK2 protein (Catalog#50432-M20B)
Species Reactivity
Mouse CHK2 / CHEK2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CHK2 / CHEK2 (rM CHK2 / CHEK2; Catalog#50432-M20B; Q9Z265; Met1-Leu546). CHK2 / CHEK2 specific IgG was purified by Mouse CHK2 / CHEK2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CHEK2 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-Cds1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CHK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HUCDS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Rad53ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CHEK2 Background Information

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Full Name
checkpoint kinase 2
Related Pathways
  • p53 Pathway
    p53 Pathway
References
  • Bogdanova N, et al. (2005) Association of two mutations in the CHEK2 gene with breast cancer. Cancer Genetics. 116(2) : 263-6.
  • Dong XY, et al. (2003) Mutations in CHEK2 associated with prostate cancer risk. The American journal of human genetics. 72(2) 270-80.
  • Massink MPG, Kooi IE, Martens JWM, Waisfisz Q, Meijers-Heijboer H. Genomic profiling of CHEK2*1100delC-mutated breast carcinomas. BMC Cancer. 2015;15:877. doi:10.1186/s12885-015-1880-y.
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