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Anti-CD97 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD97 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD97 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD97 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD97 Magnetic Beads-IP Kit Product Description

The Anti-CD97 magnetic Beads, conjugated with Anti-CD97 antibody, are used for immuneprecipitation (IP) of CD97 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD97 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD97 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD97 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD97 Antibody(11280-RP02)
Immunogen
Recombinant Human CD97 protein (Catalog#11280-H08H)
Species Reactivity
Human CD97
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD97 (rh CD97; Catalog#11280-H08H; NP_001775.2; Met 1-Gln 398). CD97 specific IgG was purified by human CD97 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD97 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD97ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TM7LN1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD97 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
Full Name
adhesion G protein-coupled receptor E5
References
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Gray JX, et al. (1996) CD97 is a processed, seven-transmembrane, heterodimeric receptor associated with inflammation. The journal of Immunology. 157 (12): 5438-47.
  • Hamann J,et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.European Journal of Immunology. 28 (5): 1701-7.
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