Anti-CD10 Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.
Price:
Size:
Number:

Anti-CD10 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD10 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD10 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD10 Magnetic Beads-IP Kit Product Description

The Anti-CD10 magnetic Beads, conjugated with Anti-CD10 antibody, are used for immuneprecipitation (IP) of CD10 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD10 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD10 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD10 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD10 Antibody(90177-T60)
Immunogen
Recombinant Rhesus CD10 / Neprilysin / MME Protein (Catalog#90177-C07H)
Species Reactivity
Rhesus CD10 / Neprilysin / MME
Source
Polyclonal Rhesus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rhesus CD10 / Neprilysin / MME ( Catalog#90177-C07H; NP_001247743.1; Tyr52-Trp750). CD10 / Neprilysin / MME specific IgG was purified by Rhesus CD10 / Neprilysin / MME affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

CD10 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD1 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD1 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD1 is also associated with other biochemical processes.
Full Name
membrane metallo-endopeptidase
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
  • Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.