Anti-Cathepsin B Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Cathepsin B Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Cathepsin B Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Cathepsin B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Cathepsin B Magnetic Beads-IP Kit Product Description

The Anti-Cathepsin B magnetic Beads, conjugated with Anti-Cathepsin B antibody, are used for immuneprecipitation (IP) of Cathepsin B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cathepsin B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cathepsin B proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Cathepsin B Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Cathepsin B Antibody(10483-RP02)
Immunogen
Recombinant Human Cathepsin B / CTSB protein (Catalog#10483-H08H)
Species Reactivity
Human Cathepsin B / CTSB
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Cathepsin B / CTSB (rh Cathepsin B / CTSB; Catalog#10483-H08H; NP_001899.1; Arg18-Ile339). Cathepsin B / CTSB specific IgG was purified by Human Cathepsin B / CTSB affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Cathepsin B Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-APPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CPSBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Cathepsin B Background Information

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
Full Name
cathepsin B
References
  • Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.
  • Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.
  • Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.
  • Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.
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