Anti-Carbonic Anhydrase XIV Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Carbonic Anhydrase XIV Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Carbonic Anhydrase XIV Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Carbonic Anhydrase XIV magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Carbonic Anhydrase XIV Magnetic Beads-IP Kit Product Description

The Anti-Carbonic Anhydrase XIV magnetic Beads, conjugated with Anti-Carbonic Anhydrase XIV antibody, are used for immuneprecipitation (IP) of Carbonic Anhydrase XIV proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Carbonic Anhydrase XIV proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Carbonic Anhydrase XIV proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Carbonic Anhydrase XIV Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Carbonic Anhydrase XIV Antibody(10458-T60)
Immunogen
Recombinant Human Carbonic Anhydrase XIV / CA14 Protein (Catalog#10458-H08H)
Species Reactivity
Human Carbonic Anhydrase XIV / CA14
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase XIV / CA14 (rh Carbonic Anhydrase XIV / CA14; Catalog#10458-H08H; NP_036245.1; Met1-Met290). Carbonic Anhydrase XIV / CA14 specific IgG was purified by Human Carbonic Anhydrase XIV / CA14 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Carbonic Anhydrase XIV Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CA14ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CAXiVALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Carbonic Anhydrase XIV Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.
Full Name
carbonic anhydrase XIV
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.
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