Anti-CALML3 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CALML3 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CALML3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CALML3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CALML3 Magnetic Beads-IP Kit Product Description

The Anti-CALML3 magnetic Beads, conjugated with Anti-CALML3 antibody, are used for immuneprecipitation (IP) of CALML3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CALML3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CALML3 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CALML3 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CALML3 Antibody, Rabbit Polyclonal(12480-RP02)
Immunogen
Recombinant Human CALML3 protein (Catalog#12480-H07E)
Species Reactivity
Human CALML3
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). CALML3 specific IgG was purified by Human CALML3 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CALML3 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CLPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CALML3 Background Information

Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Research has identified the unconventional myosin-1 (Myo1) as a specific target of CALML3. The discovery of Myo1 as a specific target of CALML3 is highly significant and suggests multiple lines of further research such as investigations of the Ca2+ regulation of Myo1 and the role of the loss of CLP in epithelial differentiation, adhesion, and cancer. Cells expressing CALML3 displayed a striking increase in the number and length of myosin-1-positive filopodia and showed increased mobility in a wound healing assay.
Full Name
calmodulin-like 3
Related Pathways
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
References
  • Bennett RD, et al. (2007) Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. J Biol Chem. 282(5): 3205-12.
  • Perochon A, et al. (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie. 93(12): 2048-53.
  • Chinpongpanich A, et al. (2011) Biophysical characterization of calmodulin and calmodulin-like proteins from rice, Oryza sativa L. Acta Biochim Biophys Sin (Shanghai). 43(11): 867-76.
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