Anti-CA13 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CA13 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CA13 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CA13 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CA13 Magnetic Beads-IP Kit Product Description

The Anti-CA13 magnetic Beads, conjugated with Anti-CA13 antibody, are used for immuneprecipitation (IP) of CA13 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CA13 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CA13 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CA13 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CA13 Antibody(10461-RP02)
Immunogen
Recombinant Human Carbonic Anhydrase XIII / CA13 protein (Catalog#10461-H08E)
Species Reactivity
Human Carbonic Anhydrase XIII / CA13
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase XIII / CA13 (rh Carbonic Anhydrase XIII / CA13; Catalog#10461-H08E; NP_940986.1; Met 1-His 262). Carbonic Anhydrase XIII / CA13 specific IgG was purified by Human Carbonic Anhydrase XIII / CA13 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CA13 Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-CAXIIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FLJ37995ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MGC59868ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CA13 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.
Full Name
carbonic anhydrase XIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
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