Anti-Alkaline Phosphatase/ALPL Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Alkaline Phosphatase/ALPL Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Alkaline Phosphatase/ALPL Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Alkaline Phosphatase/ALPL magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Alkaline Phosphatase/ALPL Magnetic Beads-IP Kit Product Description

The Anti-Alkaline Phosphatase/ALPL magnetic Beads, conjugated with Anti-Alkaline Phosphatase/ALPL antibody, are used for immuneprecipitation (IP) of Alkaline Phosphatase/ALPL proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Alkaline Phosphatase/ALPL proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Alkaline Phosphatase/ALPL proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Alkaline Phosphatase/ALPL Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Alkaline Phosphatase/ALPL Antibody(100356-R077)
Immunogen
A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL
Species Reactivity
Human Alkaline Phosphatase / ALPL
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Alkaline Phosphatase/ALPL Magnetic Beads Immunoprecipitation (IP) Kit: Synonyms

Anti-AP-TNAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-APTNAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HOPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNSALPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Alkaline Phosphatase/ALPL Background Information

Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
Full Name
alkaline phosphatase, liver/bone/kidney
References
  • Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.
  • Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.
  • Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.
  • Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
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