Anti-PRMT6 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-PRMT6 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-PRMT6 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PRMT6 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-PRMT6 Magnetic Beads-IP Kit Product Description

The Anti-PRMT6 magnetic Beads, conjugated with Anti-PRMT6 antibody, are used for immuneprecipitation (IP) of PRMT6 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PRMT6 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PRMT6 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-PRMT6 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-PRMT6 Antibody(11313-RP02)
Immunogen
Recombinant Human PRMT6 protein (Catalog#11313-H18H)
Species Reactivity
Human PRMT6 / HRMT1L6
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PRMT6 (rh PRMT6; Catalog#11313-H18H; NP_060607.2; Met 1-Asp 375). PRMT6 specific IgG was purified by human PRMT6 affinity chromatography .
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PRMT6 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-HRMT1L6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PRMT6 Background Information

Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.
Full Name
protein arginine methyltransferase 6
References
  • Hyllus D, et al., 2007, Genes Dev. 21(24): 3369-80.
  • Lakowski, TM. et al., 2008, J Biol Chem. 283 (15): 10015-25. 
  • Michaud-Levesque, J. et al., 2009, J Biol Chem. 284 (32): 21338-46.
  • Harrison, MJ. et al., 2010, Nucleic acids Res. Jan 4.
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