Anti-ICAM-2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-ICAM-2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-ICAM-2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-ICAM-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-ICAM-2 Magnetic Beads-IP Kit Product Description

The Anti-ICAM-2 magnetic Beads, conjugated with Anti-ICAM-2 antibody, are used for immuneprecipitation (IP) of ICAM-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ICAM-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ICAM-2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-ICAM-2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-ICAM-2 Antibody(10332-RP02)
Immunogen
Recombinant Human ICAM-2 / CD102 protein (Catalog#10332-H08H)
Species Reactivity
Human ICAM-2 / CD102
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ICAM-2 / CD102 (rh ICAM-2 / CD102; Catalog#10332-H08H; NP_777596.2; Met 1-Gln 223). ICAM-2 / CD102 specific IgG was purified by Human ICAM-2 / CD102 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-ICAM-2 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD102ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

ICAM-2 Background Information

Intercellular adhesion molecule 2 (ICAM-2, CD12), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.
Full Name
intercellular adhesion molecule 2
References
  • Weber KS, et al. (2004) Sialylation of ICAM-2 on platelets impairs adhesion of leukocytes via LFA-1 and DC-SIGN. Inflammation. 28(4): 177-88.
  • Tanaka H, et al. (2004) ICAM-2 gene therapy for peritoneal dissemination of scirrhous gastric carcinoma. Clin Cancer Res. 10(14): 4885-92.
  • Younis AI, et al. (2005) Intercellular adhesion molecule-2 (ICAM-2) in experimental trichinosis. J Egypt Soc Parasitol. 35(3): 1019-26.
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