Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
The skin is the largest organ of the body, with a total area of about 20 square feet. In mammals, the skin is an organ of the integumentary system made up of multiple layers of ectodermal tissue, and guards the underlying muscles, bones, ligaments and internal organs. Skin has three layers: The outermost layer of skin is epidermis which provide a waterproof barrier and create a color. The dermis, beneath the epidermis, contains tough connective tissue, hair follicles, and sweat glands. The deeper subcutaneous tissue (hypodermis) is made of fat and connective tissue. Skin cancer including malignant melanoma, basal cell and squamous cell. Basal and squamous cell more common, treatment is very effective. Malignant melanoma can be difficult to treat. Early diagnosis and treatment can improve survival in patients with melanoma. A variety of target molecules expressed on skin tissue is used for the clinical diagnosis of skin disease, skin tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect skin disease.
Different species of skin slices as follows: