Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
Paraffin (paraffin section) is the most widely used tabletting techniques in histology. Not only for observe morphology of normal tissue, but also for study morphology changes of tissue in pathology. It has been quite widely used in many areas of research in other filed. Paraffin method includes drawn, fixed, washed and dehydrated, transparent, wax dipped, embedding, sectioning and sticky films, dewaxing, staining, dehydration, transparent, mounted and other steps. General the tissue from drawn and fixed to mounted for slide specimens take several days, but the specimen can be stored for a long time.
Cytoplasm staining as follows:
|CEACAM1 / CD66a: 10822-MM02
Immunochemical Staining of human CEACAM1 in human liver with Mouse Monoclonal Antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to bile canaliculus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
|CD147 / EMMPRIN / Basigin: 10186-R125
Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
|MSH6 / GTBP: 100973-T08
Immunochemical staining of human MSH6 in human colon carcinoma with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human S100B in human malignant melanoma with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).