Immunohistochemistry / IHC Antibody-Colon Tissue

Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.

The colon is also called the large intestine. It is the last part of the digestive system of vertebrates. Here the water is absorbed; the body remaining waste is stored here before excreted. In humans, the large intestine of about 1.5 meters long, which is one fifth of the entire length of the gastrointestinal tract. In mammals, the colon consists of four sections: the ascending colon, the transverse colon, the descending colon, and the sigmoid colon. The large intestine takes about 16 hours to compelet the digestion of the food. It removes water and any remaining absorbable nutrients from the food before sending the indigestible matter to the rectum. A variety of target molecules expressed on colon tissue is used for the clinical diagnosis of colont disease, colon tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect colon disease.

Different species of colon slices as follows:

Human

CDH17: 11360-R029

Immunochemical staining of human CDH17 in human colon with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.
CES3: 11129-R033

Immunochemical staining of human CES3 in human colon with rabbit monoclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
CTNNB1: 11279-RP02

Immunochemical staining of human CTNNB1 in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.
DMBT1: 11678-RP02

Immunochemical staining of human DMBT1 in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
GPA33: 11277-RP02

Immunochemical staining of human GPA33 in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.

Ferret

ferCD20: 60004-RP02

Immunochemical staining of ferret DMBT1 in ferret colon carcinoma with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).