IL-6R Protein, Human, Recombinant (Fc Tag)

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IL-6R Protein, Human, Recombinant (Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
Measured by its ability to enhance the activity of IL6 on M1 mouse myeloid leukemia cells.
The ED50 for this effect is typically 30-125 ng/mL in the presence of 100 ng/mL of recombinant human IL6.
Protein Construction
A DNA sequence encoding the human IL6R (NP_000556.1) (Met1-Pro365) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Pro 20
Molecule Mass
The recombinant human IL6R consists of 464 amino acids and predicts a molecular mass of 52.2 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-6R Protein, Human, Recombinant (Fc Tag): Images

IL-6R Protein, Human, Recombinant (Fc Tag): Alternative Names

CD126 Protein, Human; gp80 Protein, Human; IL-6R Protein, Human; IL-6R-1 Protein, Human; IL-6RA Protein, Human; IL6Q Protein, Human; IL6RA Protein, Human; IL6RQ Protein, Human

IL-6R Background Information

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
Full Name
interleukin 6 receptor
References
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
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