IL-15 Matched ELISA Antibody Pair Set,Human

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IL-15 Matched ELISA Antibody Pair Set,Human General Information

Product name
IL-15 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human IL-15
Assay range
9.38-600 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human IL15/IL-15/Interleukin 15 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10360-R112)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human IL15/IL-15/Interleukin 15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.7 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10360-R111)
3. Standard Each vial contains 45 ng of recombinant Human IL15/IL-15/Interleukin 15. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Product overview
This IL-15 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human IL-15 . It contains Human IL-15 capture antibody, Human IL-15 detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human IL-15 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

IL-15 Matched ELISA Antibody Pair Set,Human Images

IL-15 Matched ELISA Antibody Pair Set,Human: Alternative Names

IL-15 Matched ELISA Antibody Pair Set, Human; Interleukin 15 Matched ELISA Antibody Pair Set, Human

IL-15 Background Information

The protein encoded by IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported.
Full Name
interleukin 15
References
  • Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md : 1950). 2015;195(3):901-923.
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