IL-1 beta Protein, Mouse, Recombinant

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IL-1 beta Protein, Mouse, Recombinant: Product Information

Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 6-30 ng/mL..
2. Measured by its ability to bind human IL1R1 in a functional ELISA.
3. Measured by its ability to bind mouse IL1R1 in a functional ELISA.
Protein Construction
A DNA sequence encoding the mature form of mouse IL1β (P10749) (Val 118-Ser 269) was expressed, with an initial Met at the C-terminus.
Accession#
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse IL1β consists of 153 amino acids and has a predicted molecular mass of 17.5 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-1 beta Protein, Mouse, Recombinant: Images

IL-1 beta Protein, Mouse, Recombinant: Alternative Names

Il-1b Protein, Mouse; IL-1beta Protein, Mouse

IL-1 beta Background Information

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Full Name
interleukin 1, beta
References
  • Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
  • Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
  • Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
  • Differential Patterns of Secreted Frizzled-Related Protein 4 (SFRP4) in Adipocyte Differentiation: Adipose Depot Specificity
    Author
    Guan, H;Zhang, Y;Gao, S;Bai, L;Zhao, S;Cheng, XW;Fan, J;Liu, E;
    Year
    2018
    Journal
    Cell. Physiol. Biochem.
    Application
    cell-based
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