I-309 Protein, Human, Recombinant (Fc Tag)

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I-309 Protein, Human, Recombinant (Fc Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mature form of human CCL1 (NP_002972.1) (Lys 24-Lys 96) was fused with the Fc region of human IgG1 at the N-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 20
Molecule Mass
The recombinant human Fc/CCL1 is a disulfide-linked homodimeric protein. The reduced monomer consists of 310 amino acids and predicts a molecularmass of 35 kDa. As a result of glycosylation, the rhCCL1/Fc monomer migrates as an approximately 38-42 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

I-309 Protein, Human, Recombinant (Fc Tag): Images

I-309 Protein, Human, Recombinant (Fc Tag): Alternative Names

I-309 Protein, Human; P500 Protein, Human; SCYA1 Protein, Human; SISe Protein, Human; TCA3 Protein, Human

I-309 Background Information

CCL1 or chemokine (C-C motif) ligand 1, also known as I-39 or TCA-3, is a member of the chemokine (C-C motif) ligand family. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands (CCL)-1 to 28. I-39/CCL1/TCA-3 interacts with the G protein-linked transmembrane chemokine receptors CCR8, and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that I-39/CCL1/TCA-3 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, I-39/CCL1/TCA-3 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. I-39/CCL1/TCA-3 induce significant levels of LTC4 from elicited eosinophils.
Full Name
chemokine (C-C motif) ligand 1
References
  • Miller MD, et al. (1992) The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci. 89 (7): 2950-4.
  • Roos RS, et al. (1997) Identification of CCR8, the receptor for the human CC chemokine I-309. J Biol Chem. 272 (28): 17251-4.
  • Oliveira SH, et al. (2002) Increased responsiveness of murine eosinophils to MIP-1beta (CCL4) and TCA-3 (CCL1) is mediated by their specific receptors, CCR5 and CCR8. J Leukoc Biol. 71(6): 1019-25.
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