Vimentin Protein, Human, Recombinant (His Tag)

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Vimentin Protein, Human, Recombinant (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human VIM (Met 1-Glu466) (P08670) was expressed, with a C-terminal polyhistidine tag.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The secreted recombinant human VIM consists of 476 amino acids and predicts a molecular mass of 55 KDa. The apparent molecular mass of the protein is approximately 56 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Vimentin Protein, Human, Recombinant (His Tag): Images

Vimentin Protein, Human, Recombinant (His Tag): Alternative Names

CTRCT30 Protein, Human; HEL113 Protein, Human

Vimentin Background Information

Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
Full Name
vimentin
References
  • Russell RL, et al. (2001) Uridine phosphorylase association with vimentin. Intracellular distribution and localization. J Biol Chem. 276(16):13302-7.
  • Moinova, et al. (2012) Aberrant Vimentin Methylation is Characteristic of Upper GI Pathologies. Cancer Epidemiology Biomarkers Prev. 21(4):594-600.
  • Leader M, et al. (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology. 11(1):63-72.
  • Extracellular vimentin modulates human dendritic cell activation
    Author
    Yu, MB;Guerra, J;Firek, A;Langridge, WHR;
    Year
    2018
    Journal
    Mol. Immunol.
    Application
    cell-based (DC activation)
  • Click RNA for Rapid Capture and Identification of Intracellular MicroRNA Targets
    Author
    Zhang, P;Fu, H;Du, S;Wang, F;Yang, J;Cai, W;Liu, D;
    Year
    2019
    Journal
    Anal. Chem.
    Application
    binding
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