IL-8 ELISA Kit, Human

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IL-8 ELISA Kit, Human: General Information

Product name
IL-8 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
2.21 pg/mL
Assay range
2.5-160 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-8
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL8 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 81.82 149.44 293.58
SD 4.22 2.58 8.43
CV(%) 5.2% 1.7% 2.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 81.59 158.99 311.09
SD 3.68 9.26 15.19
CV(%) 4.5% 5.8% 4.9%
Recovery
The recovery of Human CXCL8 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
90
Range
72-109%
Linearity
Supernatant
  Recovery of detected
1:2 97%
1:4 103%
1:8 109%
1:16 112%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Standard Dilution Buffer
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
Product overview
This IL-8 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 protein in Cell supernatant . It contains recombinant Human IL-8, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-8 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL8. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-8 ELISA Kit, Human: Alternative Names

GCP-1 ELISA Kit, Human; GCP1 ELISA Kit, Human; IL-8 ELISA Kit, Human; IL8 ELISA Kit, Human; Interleukin-8 ELISA Kit, Human; LECT ELISA Kit, Human; LUCT ELISA Kit, Human; LYNAP ELISA Kit, Human; MDNCF ELISA Kit, Human; MONAP ELISA Kit, Human; NAF ELISA Kit, Human; NAP-1 ELISA Kit, Human; NAP1 ELISA Kit, Human

IL-8 Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Full Name
chemokine (C-X-C motif) ligand 8
References
  • Mukaida N. (2003) Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases. Am J Physiol Lung Cell Mol Physiol. 284(4): L566-77.
  • Brat DJ, et al. (2005) The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro Oncol. 7(2): 122-33.
  • Bendrik C, et al. (2009) Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. J Immunol. 182(1): 371-8.
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