CSF1R ELISA Kit, Human

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CSF1R ELISA Kit, Human: General Information

Product name
CSF1R ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
30 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Human CSF1R
Interference
Recombinant human CSF1 prepared at 50 ng/mL were assayed and exhibited no interference.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.13 0.31 0.65
SD 0.01 0.01 0.03
CV(%) 5.1% 4.8% 4.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.15 0.30 0.68
SD 0.02 0.02 0.05
CV(%) 10.3% 5.6% 7.5%
Recovery
The recovery of Human CSF1R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
94
Range
92 -99%
Linearity
Serum
  Recovery of detected
1:2 90%
1:4 93%
1:8 95%
1:16 89%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CSF1R ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CSF1R protein in Serum . It contains recombinant Human CSF1R, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CSF1R ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human MCSFR. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Recombinant human CSF1 prepared at 50 ng/mL were assayed and exhibited no interference.

CSF1R ELISA Kit, Human: Alternative Names

C-FMS ELISA Kit, Human; CD115 ELISA Kit, Human; CSF-1R ELISA Kit, Human; CSFR ELISA Kit, Human; FIM2 ELISA Kit, Human; FMS ELISA Kit, Human; HDLS ELISA Kit, Human; M-CSF-R ELISA Kit, Human; MCSF Receptor ELISA Kit, Human

CSF1R Background Information

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.
Full Name
colony stimulating factor 1 receptor
References
  • Sherr CJ. (1990) The colony-stimulating factor 1 receptor: pleiotropy of signal-response coupling. Lymphokine Res. 9(4): 543-8.
  • Kacinski BM. (1997) CSF-1 and its receptor in breast carcinomas and neoplasms of the female reproductive tract. Mol Reprod Dev. 46(1): 71-4.
  • Sapi E, et al. (1999) The role of CSF-1 in normal and neoplastic breast physiology. Proc Soc Exp Biol Med. 220(1): 1-8.
  • Sapi E. (2004) The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update. Exp Biol Med (Maywood). 229(1): 1-11.
  • Bonifer C, et al. (2008) The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis. Front Biosci. 13: 549-60.
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