With the rapid development of biopharmaceutical research in recent years, more and more researchers hope to validate a large number of screening antibody samples in a short time. In order to meet the increasing demand for high-throughput and rapid-expression of antibodies, Sino Biological provides high-throughput recombinant antibody expression and purification services that has combined professional high-throughput gene synthesis, vector construction, and optimized technology of transient antibody expression. Making full use of superior mammalian cell expression platforms, Sino Biological can provide services of rapid production of recombinant antibodies in HEK293/CHO cells.
Gene synthesis and codon optimization
|2-3 weeks||• 50 μg ~ 100 μg
• 101 μg ~ 500 μg
• 501 μg ~ 1 mg
• >1 mg ~ 5 mg
|• Purified antibody
• Expression vector
|• Expression vector construction
• Plasmid sequencing
• Plasmid production
Expression & purification
|• Transient transfection of HEK293 / CHO cells
• Purify the anitbody by one-step affinity purification
|• Analysis by SDS-PAGE and UV|
1.Client needs to provide the antibody sequence or plasmid.
2.A standard service includes: antibody concentration detected by A280 and purity evaluated by SDS-PAGE. If other detection services such as SEC-HPLC and endotoxin test are required, please indicate it in the request form.