Anti-Her2 / ERBB2 Antibody

1/2
Price:
Size:
Number:

Anti-Her2 / ERBB2 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Her2 / ERBB2 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Rat
Specificity
Rat Her2 / ERBB2
Immunogen
Recombinant Rat HER2 / ErbB2 Protein (Catalog#80079-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat HER2 / ErbB2 (rR HER2 / ErbB2; Catalog#80079-R08H; AAH61863.1; Met4-Thr656). HER2 / ErbB2 specific IgG was purified by Rat HER2 / ErbB2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Her2 / ERBB2 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Her2 / ERBB2 Antibody (Rabbit Polyclonal antibody) Images

rat Erbb2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-rat Erbb2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat Erbb2 rabbit polyclonal antibody,at 1:70 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 138 kDa

Observed band size: 190 kDa

Anti-Erbb2 rabbit polyclonal antibody at 1:500 dilution

Lane A: SKBR3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:138 kDa

Observed band size:190 kDa

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait