Anti-GAPDH Antibody

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Anti-GAPDH Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-GAPDH Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human GAPDH
Immunogen
Recombinant Human GAPDH Protein (Catalog#10094-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human GAPDH (rh GAPDH; Catalog#10094-H07E; P04406; Met1-Glu335). GAPDH specific IgG was purified by Human GAPDH affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GAPDH Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GAPDH Antibody (Rabbit Polyclonal antibody) Images

GAPDH was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

Lane C:0.5 mg Hela Whole Cell Lysate

4 µL anti-GAPDH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-GAPDH rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 whole cell lysate

Lane B: Jurkat whole cell lysate

Lane C: Hela whole cell lysate

Lane D: A431 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:36 kDa

(We are unsure as to the identity of these extra bands.)

Anti-GAPDH Antibody: Alternative Names

Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody

GAPDH Background Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Full Name
glyceraldehyde-3-phosphate dehydrogenase
References
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
  • Uric acid upregulates the adiponectin‑adiponectin receptor 1 pathway in renal proximal tubule epithelial cells
    Author
    Yang, Q;Fu, C;Xiao, J;Ye, Z;
    Year
    2017
    Journal
    Molecular Medicine Reports
    Application
    WB
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