Flow Cytometry (FCM) /FACS | Flow Cytometer

What Happens in a Flow Cytometer?

1. Fluidics: Cells in suspension flow in single‐file through an illuminated volume where they scatter light and emit fluorescence

• When conditions are right, the sample fluid flows in a central core that does not mix with the sheath fluid
• The introduction of a large volume into a small volume in such a way that it becomes "focused" along an axis is called Hydrodynamic Focusing

2. Optics: Light and fluorescence is collected, filtered and converted

3. Electronics: Light signals were converted to digital values that are stored on a computer