Flow Cytometry (FCM) / FACS Antibody Verified by Multiple Cell Samples

The Purposes of Multi-Cell Line Verification:

1. Due to cell differentiation, antigen expression may vary in different tissues derived cell lines. With an antibody validation through multiple cell lines will further clarify the antibody specificity;
2. Some cell lines do not express the corresponding antigen, which is the natural "negative cells", by verifying negative cells, antibody specificity would be more clear;
3. Under pathological conditions, some special cells such as tumor cells, whose specific antigen expression relative to normal cells from same resources would change significantly. By verifying that of the tumor cell lines, will further define the accuracy of antibody specificity;
4. There are many cell lines available in Sino-Biological Inc. to assure prospected specificity of our antibodies.

For example,

Protein TP53 get higher expression in human brain, breast, kidney and colon tissues. These tissue-derived cell lines such as SF-268 (brain), HCC-1143 (breast), KM-12 (colon), HEK-293 (kidney), NCI-H23 high (lung), etc. have higher TP53 expression. (Source: Proteomics DB)

Flow Cytometry / FACS Background

Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection and protein engineering.