Flow Cytometry (FCM) /FACS | Antibody Host & Clone

SBI Antibody Production Flowchart

Second Generation Technology for the Production of Rabbit Monoclonal Antibody

In contrast to rabbit hybridoma technology, which is the first generation technology for the production of monoclonal rabbit antibody, Sino Biological Inc. (SBI) has built up a unique rabbit monoclonal antibody production platform that is based on our unparalleled expertise in phage display antibody library technology, which has otherwise been called the second generation technology for the production of monoclonal rabbit antibody.

We generate rabbit antibody libraries and select high-affinity antigen-specific antibodies by phage display methodology. Usually, two or more rabbits of the b9 allotype are immunized with one antigen, total RNA from spleen lymphocytes and bone marrow cells are purified, and then the first-strand cDNAs are synthesized. cDNA from spleen and bone marrow samples derived from multiple groups of animals immunized by different antigens can be pooled to make a unified library for all the antigens. Chimeric rabbit/human Fab libraries are made in our display phagemid vectors by fusing rabbit Vκ/Vλ to human Cκ and rabbit VH to human CH1 of human IgG1.

The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies and monoclonal antibodies that combine high specificity with high avidity and affinity. In addition, rabbits are evolutionarily distant from mice and rats. As a consequence, epitopes conserved between rodent and human antigens that are invisible to rodent monoclonal antibodies (and also human monoclonal antibodies generated from transgenic mice with human immunoglobulin genes) can often be recognized by rabbit antibodies. Rabbit monoclonal antibodies have overcome this limitation, providing access to defined reagents of infinite supply from the rabbit antibody repertoire. Rabbit monoclonal antibodies generated by phage display offer additional advantages due to the fact that the phenotype (protein) and genotype (cDNA) are selected at the same time. Also, in comparison with rabbit hybridoma technology, our phage-display platform can produce much more antibody clones at a time.

The Benefit of Rabbit Monoclonal Antibody

First Vs. Second Generation Rabbit Monoclonal Antibody

  The First Generation Rabbit Mab The Second Generation Rabbit Mab
Technology Rabbit Hybridoma Phage Display
Antibody Library Limited Broad
Screening Throughput Lower Larger
Epitope Coverage Smaller Broad
Success Rate Lower Higher
Antibody Yield Lower Higher
Stability of Clones Lower Higher
Accessibility to Antibody Engineering Difficult Easy

Mouse Vs. Rabbit Monoclonal Antibody

  The First Generation Rabbit Mab The Second Generation Rabbit Mab
Technology Rabbit Hybridoma Phage Display
Antibody Library Limited Broad
Screening Throughput Lower Larger
Epitope Coverage Smaller Broad
Success Rate Lower Higher
Antibody Yield Lower Higher
Stability of Clones Lower Higher
Accessibility to Antibody Engineering Difficult Easy

SBI have constructed a large number of immunized rabbit antibody libraries. Rabbit MAb primary antibodies typically provide more specific and sensitive detection of their target protein with low background.