Flow Cytometry / FACS Antibody | Receptor

Background

Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection andprotein engineering.
Receptor proteins are located within the cell surface membrane, nucleus membrane or other cellular organelle membrane. They can bind to corresponding ligands to initiate cellular signaling pathways. For cell surface receptors, such as receptor tyrosine kinases, interleukin receptors and receptors of growth factors, they are usually subdivided into three domains, extracellular domain, transmembrane domain and intracellular domain.