FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag)

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FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind human IgG1 in a functional ELISA.
2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio
3. Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag),Biotinylated(Cat. CT009-H08H-B) bound to Human IgG1 was 10-7-10-9M.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 24 & Ile 21
Molecule Mass
The recombinant heterodimer of human FCGRT/B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of rh FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): Images

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag): Alternative Names

alpha-chain Protein, Human; FCRN Protein, Human

FCGRT & B2M Heterodimer Background Information

Full Name
Fc fragment of IgG, receptor, transporter, alpha
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