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Von Willebrand Factor/vWF ELISA Kit, Human

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Von Willebrand Factor/vWF ELISA Kit, Human: General Information

Product name
Von Willebrand Factor/vWF ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
155.29 pg/mL
Assay range
312.5-20000 pg/mL
Specificity
Recognizes both recombinant and natural Human Von Willebrand Factor/vWF
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,074.19 4,023.55 8,366.13
SD 123.01 248.21 502.56
CV(%) 5.9% 6.2% 6.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,216.70 3,880.11 8,496.22
SD 199.73 306.14 340.03
CV(%) 9.0% 7.9% 4.0%
Recovery
The recovery of Human vWF spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
108
Range
107 -109%
Linearity
Serum
  Recovery of detected
1:2 90%
1:4 100%
1:8 100%
1:16 104%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This Von Willebrand Factor/vWF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Von Willebrand Factor/vWF protein in Serum . It contains recombinant Human Von Willebrand Factor/vWF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Von Willebrand Factor/vWF ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Von Willebrand Factor/vWF ELISA Kit, Human: Alternative Names

F8VWF ELISA Kit, Human; Von Willebrand Factor ELISA Kit, Human; VWD ELISA Kit, Human

Von Willebrand Factor/vWF Background Information

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.
Full Name
von Willebrand factor
References
  • Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.
  • Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.
  • Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.
  • Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.
  • Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.
  • Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
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