TNFR2/TNFRSF1B ELISA Kit, Mouse

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TNFR2/TNFRSF1B ELISA Kit, Mouse: General Information

Product name
TNFR2/TNFRSF1B ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
4.1 pg/mL
Assay range
6.25-400 pg/mL
Specificity
Recognizes both recombinant and natural Mouse TNFR2/TNFRSF1B
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.07 0.15 0.29
SD 0.00 0.00 0.01
CV(%) 4.4% 2.7% 1.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.08 0.16 0.32
SD 0.00 0.01 0.03
CV(%) 5.3% 5.9% 7.8%
Recovery
The recovery of Mouse TNFRSF1B spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
76.69545745361481
Range
75-78%
Linearity
Serum
  Recovery of detected
1:2 83%
1:4 121%
1:8 114%
1:16 80%
Supernatant
  Recovery of detected
1:2 85%
1:4 79%
1:8 80%
1:16 77%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TNFR2/TNFRSF1B ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNFR2/TNFRSF1B protein in Serum . It contains recombinant Mouse TNFR2/TNFRSF1B, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TNFR2/TNFRSF1B ELISA Kit, Mouse: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural mouse TNFRSF1B. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity and interference. No cross-reactivity or interference was observed.

TNFR2/TNFRSF1B ELISA Kit, Mouse: Synonyms

CD120b ELISA Kit, Mouse; p75 ELISA Kit, Mouse; TNF-alphaR2 ELISA Kit, Mouse; TNF-R-II ELISA Kit, Mouse; TNF-R2 ELISA Kit, Mouse; TNF-R75 ELISA Kit, Mouse; TNFalpha-R2 ELISA Kit, Mouse; TNFBR ELISA Kit, Mouse; Tnfr-1 ELISA Kit, Mouse; Tnfr2 ELISA Kit, Mouse; TNFR80 ELISA Kit, Mouse; TNFRII ELISA Kit, Mouse

TNFR2/TNFRSF1B Background Information

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Full Name
tumor necrosis factor receptor superfamily, member 1b
Research Areas
References
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
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