TNF-alpha ELISA Kit, Human
Cat: KIT10602
TNF-alpha ELISA Kit, Human: General Information
Product name
TNF-alpha ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
13.06 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Human TNF-alpha
Interference
Preparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFa control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 277.00 | 595.00 | 1,115.00 |
| SD | 10.66 | 14.44 | 30.07 |
| CV(%) | 3.9% | 2.4% | 2.7% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 269.00 | 587.00 | 1,091.00 |
| SD | 20.77 | 43.24 | 66.66 |
| CV(%) | 7.7% | 7.4% | 6.1% |
Recovery
The recovery of Human TNF spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 86 |
| Range |
| 73 -95% |
Linearity
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 84% |
| 1:4 | 109% |
| 1:8 | 91% |
| 1:16 | 84% |
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TNF-alpha ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TNF-alpha protein in Cell supernatant . It contains recombinant Human TNF-alpha, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
TNF-alpha ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TNFa. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
TNF-alpha ELISA Kit, Human: Alternative Names
DIF ELISA Kit, Human; TNF-alpha ELISA Kit, Human; TNFA ELISA Kit, Human; TNFSF2 ELISA Kit, Human
TNF-alpha Background Information
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
References
- Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
- Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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Roles of extrahepatic lipolysis and the disturbance of hepatic fatty acid metabolism in TNF-α -induced hepatic steatosisAuthorYang, R;Guan, MJ;Zhao, N;Li, MJ;Zeng, T;Year2018JournalToxicologyApplicationcell-based; ELISA
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LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axisAuthorTong, P;Peng, QH;Gu, LM;Xie, WW;Li, WJ;Year2018JournalExp. Mol. Pathol.
Related Pathways
-
Death Receptor Signaling -
NF-kB (NFkB) Pathway -
IL17 signaling pathway
