TIM-3 ELISA Kit, Human

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TIM-3 ELISA Kit, Human: General Information

Product name
TIM-3 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
9.29 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Human TIM-3
Interference
Preparations of GST-Xa-Ab1/42 at 20 ng/mL in a mid-range recombinant human TIMD3 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.30 0.66 1.39
SD 0.01 0.03 0.04
CV(%) 4.6% 4.3% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.26 0.60 1.29
SD 0.02 0.06 0.09
CV(%) 7.4% 9.9% 7.2%
Recovery
The recovery of Human HAVCR2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
112
Range
94-128%
Linearity
Serum
  Recovery of detected
1:2 99%
1:4 96%
1:8 96%
1:16 82%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TIM-3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TIM-3 protein in Serum . It contains recombinant Human TIM-3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TIM-3 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human TIMD3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TIM-3 ELISA Kit, Human: Synonyms

CD366 ELISA Kit, Human; HAVcr-2 ELISA Kit, Human; HAVCR2 ELISA Kit, Human; KIM-3 ELISA Kit, Human; Tim-3 ELISA Kit, Human; TIM3 ELISA Kit, Human; TIMD-3 ELISA Kit, Human; TIMD3 ELISA Kit, Human

TIM-3 Background Information

Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In tumor rejection model, soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
Full Name
hepatitis A virus cellular receptor 2
Research Areas
References
  • Geng H, et al. (2006) Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3): 1411-20.
  • Anderson AC, et al. (2006) TIM-3 in autoimmunity. Curr Opin Immunol. 18(6): 665-9.
  • Anderson DE. (2007) TIM-3 as a therapeutic target in human inflammatory diseases. Expert Opin Ther Targets. 11(8): 1005-9.
  • Pan HF, et al. (2010) TIM-3 as a new therapeutic target in systemic lupus erythematosus. Mol Biol Rep. 37(1): 395-8.
  • Soluble Tim3 detection by time-resolved fluorescence immunoassay and its application in membranous nephropathy
    Author
    Chen, M;Wang, L;Wang, Y;Zhou, X;Liu, X;Chen, H;Huang, B;Hu, Z;
    Year
    2020
    Journal
    J. Clin. Lab. Anal.
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