NGF ELISA Kit, Human

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NGF ELISA Kit, Human: General Information

Product name
NGF ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
1.81 pg/mL
Assay range
3.13-200 pg/mL
Specificity
Recognizes both recombinant and natural Human NGF
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human β-NGF control were assayed for interference. No significant interference was observed. High significant interference were observed within standard curve of recombinant mouse β-NGF.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 41.41 72.96 138.46
SD 0.89 1.44 4.37
CV(%) 2.1% 2.0% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 38.31 68.41 118.19
SD 1.57 2.37 4.52
CV(%) 4.1% 3.5% 3.8%
Recovery
The recovery of Human NGF spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
83
Range
73-89%
Linearity
Supernatant
  Recovery of detected
1:2 110%
1:4 115%
1:8 105%
1:16 86%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This NGF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human NGF protein in Cell supernatant . It contains recombinant Human NGF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

NGF ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human β-NGF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. High cross-reactivity were observed within standard curve of recombinant mouse β-NGF.

NGF ELISA Kit, Human: Synonyms

Beta-NGF ELISA Kit, Human; HSAN5 ELISA Kit, Human; NGFB ELISA Kit, Human

NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
References
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
  • Nerve Growth Factor Gene Delivery across the Blood–Brain Barrier to Reduce Beta Amyloid Accumulation in AD Mice
    Author
    Rodrigues, B;Kanekiyo, T;Singh, J;
    Year
    2020
    Journal
    Mol. Pharmaceutics
    Application
    Elisa
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