MCP-1/CCL2 ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

MCP-1/CCL2 ELISA Kit, Human: General Information

Product name
MCP-1/CCL2 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Urine
Sensitivity
0.84 pg/mL
Assay range
3.44-220 pg/mL
Specificity
Recognizes both recombinant and natural Human MCP-1/CCL2
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human CCL2 control were assayed for interference. No significant interference was observed. Interference was observed with recombinant human MCP-2 at concentrations ≥ 1.5625 ng/mL.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 29.00 54.00 96.00
SD 1.77 2.24 4.85
CV(%) 6.1% 4.1% 5.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 26.00 54.00 96.00
SD 3.05 2.41 8.28
CV(%) 12.0% 4.0% 9.0%
Recovery
The recovery of Human CCL2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
119
Range
110-128%
Sample
urine (n=3)
Average % Recovery
91
Range
88-95%
Linearity
Serum
  Recovery of detected
1:2 106%
1:4 94%
1:8 85%
1:16 71%
Urine
  Recovery of detected
1:2 102%
1:4 83%
1:8 76%
1:16 86%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This MCP-1/CCL2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP-1/CCL2 protein in Serum,Urine . It contains recombinant Human MCP-1/CCL2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

MCP-1/CCL2 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CCL2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

MCP-1/CCL2 ELISA Kit, Human: Synonyms

GDCF-2 ELISA Kit, Human; HC11 ELISA Kit, Human; HSMCR30 ELISA Kit, Human; MCAF ELISA Kit, Human; MCP-1 ELISA Kit, Human; MCP1 ELISA Kit, Human; SCYA2 ELISA Kit, Human; SMC-CF ELISA Kit, Human

MCP-1/CCL2 Background Information

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection [7–9]. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
Full Name
chemokine (C-C motif) ligand 2
Research Areas
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Vásquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.