IL17 ELISA Kit, Human

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IL17 ELISA Kit, Human: General Information

Product name
IL17 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
0.58 pg/mL
Assay range
1.88-120 pg/mL
Specificity
Recognizes both recombinant and natural Human IL17
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL17A control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 22.14 44.67 85.98
SD 0.80 1.21 3.28
CV(%) 3.6% 2.7% 3.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 21.90 45.53 84.03
SD 0.60 1.44 1.16
CV(%) 2.7% 3.2% 1.4%
Recovery
The recovery of Human IL17A spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
102
Range
99-108%
Linearity
Supernatant
  Recovery of detected
1:2 102%
1:4 105%
1:8 106%
1:16 116%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL17 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL17 protein in Cell supernatant . It contains recombinant Human IL17, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL17 ELISA Kit, Human: Images

This assay recognizes both recombinant and natural Human IL17A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 2.3% with 50 ng/mL of recombinant Rat IL17F.
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

IL17 ELISA Kit, Human: Synonyms

CTLA-8 ELISA Kit, Human; CTLA8 ELISA Kit, Human; IL-17 ELISA Kit, Human; IL-17A ELISA Kit, Human; IL17 ELISA Kit, Human

IL17 Background Information

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Full Name
interleukin 17A
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
  • Influence of Th17/Treg balance on progression of liver cirrhosis in patients with chronic hepatitis B
    Author
    Wu, C;Dong, X;Yang, Q;Sun, Z;
    Year
    2018
    Journal
    International Journal of Clinical and Experimental Medicine
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