IL-7 ELISA Kit, Human

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IL-7 ELISA Kit, Human: General Information

Product name
IL-7 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
1.89 pg/mL
Assay range
6.25-400 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-7
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 91.00 185.00 348.00
SD 2.35 4.70 5.15
CV(%) 2.6% 2.5% 1.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 91.00 194.00 355.00
SD 5.00 19.73 36.01
CV(%) 5.5% 10.2% 10.1%
Recovery
The recovery of Human IL7 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
102
Range
98 -108%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-7 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-7 protein in Cell supernatant . It contains recombinant Human IL-7, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-7 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL7. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-7 ELISA Kit, Human: Synonyms

IL-7 ELISA Kit, Human; Interleukin-7 ELISA Kit, Human

IL-7 Background Information

IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.
Full Name
interleukin 7
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes.
  • J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses. Immunity. 30 (3):447-57.
  • Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10): 2541-4.
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